Regulatory

Part:BBa_K3844002:Design

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2021-10-21)


Propane 2-monooxygenase operon transcriptional activator MimR OS=Mycolicibacterium smegmatis (strain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
    Illegal NotI site found at 1055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 231
    Illegal BglII site found at 1392
    Illegal BamHI site found at 549
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 70
    Illegal PstI site found at 1396
    Illegal NgoMIV site found at 1368
    Illegal NgoMIV site found at 1575
    Illegal AgeI site found at 492
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.


Source

Activator, DNA-binding

References

1. https://www.bioinformatics.org/sms2/rev_trans.html

2. https://www.uniprot.org/uniprot/A0QTU7.fasta